This is specific data on one of two litres of Melissa officinalis- sub species-officinalis, purchased by Kobashi
Analysis GC-MS GC 5890 - MS 5972 Column: BPX5 59.85m, 0.22mm ID, 0.25um film, with constant flow helium carrier gas@pressure:194 kPa Updated 14.05.98 by Ira for essential oils analysis. Injection Volume: .05ml of oil diluted in 2ml of pentane, then .001 of a ml injected. Oven Temperatures: Start 70ƒC, rising 3ƒC/min to 250ƒC and held for 30 min., then 300ƒC held for 1 min. Total Run Time: 92.67 minute.Library to Search C:\DATABASE\WILEY275.L 0 Initial Threshold 14, Initial Peak Width 0.020, Shoulder detectionOff	Field of Kobashi Melissa.	Grown in Eire Distilled August 96 and stored @~5ƒC. When this Melissa (Lemon Balm) was in full blossom it was harvested at 2:00 pm.. The still used is 2.5 m x 2 m, 3 to 4 layers of fresh plant material produce 4 to 48 mls. 100's of single distil-lations created the 4.3 litres produced. 10 tons of herb created 1 litre (.01% yield). At no time were pesticides used & no fertilizers or weed inhibitors were used in the last 6 months before harvest.

RT	Component	Area%	RT	Component	Area%
6.24	1-Octen -3-ol	00.71	21.40	Geranyl acetate	01.04
6.46	6-methyl-5-hebten-2-one	00.56	21.56	Beta Bourbonene	00.39
6.68	3-Octenol	00.35	21.76	Beta Cubebene	00.68
7.97	Ocimene<(Z)-Beta->	00.06	21.84	Beta Elemene	00.77
8.21	Ocimene<(E)-Beta->	01.69	23.14	Beta-Caryophyllene	12.33
8.32	Linalool	01.39	24.34	Alpha-Humulene	01.74
10.06	Cis-Rose oxide	00.16	24.61	Allo-Aromadendrene	00.41
10.48	Trans-Rose oxide	00.12	25.61	Germacrene D	12.45
11.84	Isopulegol	00.34	26.17	Alpha-Muurolene	00.80
12.16	Citronellal	07.98	26.41	Alpha-Farnesene	00.88
15.19	Citronellol	04.73	26.73	Gamma-Cadinene	00.64
15.84	Neral	13.30	27.10	Delta-Cadinene	02.15
16.28	Geraniol	03.36	27.61	Alpha-Cadinene	00.15
16.4	Methy Citronellate	01.41	29.07	Germacradien-04-ol	00.93
17.12	Geranial	17.07	29.37	Caryophyllene oxide	01.43
18.94	Methy geranate	00.21	31.53	T-cadinol	01.28
20.12	Neryl Acetate	00.04	31.66	Muurolol-Epi-Alpha	00.24
21.19	Alpha-Copaene	01.51	32.03	Alpha-Cadinol	01.84

This particular oil took 8 hours to research individual ion patterns. RT(Retention Time) As the temperture rises in the oven the oil passes through the capillary column (BPX5). The lighter chemical components (that which evaporate quicker) come out first, such as 1-Octen -3-ol which has a RT of 06.24 minutes and the heaviest last, such as Alpha-Cadinol at 32.03 minutes. It has taken us a long time to source this fine, True and unadulterated Melissa. This excerpt from Guenther-The Essential Oils, Vol.3 1949. p. 396, Krieger Publishing Company, Malabar, Florida, explains why. Yield of Oil:The yield of the volatile oil from Melissa officinalis L. is so small that the cost of production becomes almost prohibitive. The commercial oil, therefore, is seldom, if ever, pure, and consists largely of mixtures of lemongrass and citronella, or fractions thereof. In European countries it is prepared by distilling lemon oil over Melissa officinalis L. herb. The sophistication in adulteration has increased since 1949, thus the ever increasing need to source from a Purist. All Kobashi Oils are tested as above. THIS IS ONE OF THE 246 PRODUCTS KOBASHI HOLDS IN STOCK.

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